During the \u201cGenetics of\nInfectious Diseases\u201d session of the 12^{th<\/sup> Conference of the AfSHG and\nthe 1^{st<\/sup> MSHG, Dr. Djeneba Dabitao, presented the topic: \u201cGenetic\nvariations in human IL10 proximal promoter and Susceptibility to HIV infection:\nA pilot study in Mali, West Africa<\/em>\u201d.<\/p>\n\n\n\n}}

Background<\/strong>: Interleukin-10\n(IL-10) is a key anti-inflammatory cytokine that plays an important role in\nmodulating immune responses in various conditions. Several reports have identified\nsingle nucleotide polymorphisms (SNPs) within the human \/L10 proximal Promoter\nat position -1082 (G\/A), -819 (C\/T), and -592 (C\/A). The SNPs form 3 common\nhaplotypes: GCC, ACC and ATA in humans. The \/L10 haplotypes have been\nassociated with differential IL-10 expression and disease risk, mainly in\nCaucasians ar However, very little is known about their contribution to disease\nsusceptibility such as HIV-1 in Africans populations, especially those living\nin West African region. Th we conducted a pilot study in Mali to determine\nwhether \/L10 SNPs haplotypes are associated with HIV-1 infection in a Malian\npopulation as a foundation for a larger population based study. <\/p>\n\n\n\n

Methodology:<\/strong> One hundred and\nfifty-nine peripheral blood mononuclear cells were pooled from samples\ncollected from an IRB-approved protocol of the SEREFO Laboratory at the\nUniversity Clinical Research Center in Mali. Among these samples 71 (45%) were\nHIV positive and 88 (55%) were HIV negative. Genomic DNA was isolated using the\nQuiagen’s DNeasy Blood & Tissue kit and quantified using a Napodrop\nspectrophotometer. All DNA samples were then genotyped using the Taqman SNP\ngenotyping assay on the ABI 75000.<\/p>\n\n\n\n

Results and Conclusion:<\/strong> The -592 C\/A, -819\nC\/T, and -1082 A\/A alleles were the most represented in our study population,\nirrespective of the HIV status. Likewise the dominant haplotype was the ATA-low\nIL-10 producing haplotype (71%), followed by ACC (16%) and GCC ( 13%).\nInterestingly, we found a higher frequency of ATA\/ATA carreers in HIV positive\nwhen compared to HIV negative, suggesting that the ATA-low IL-10 producing\nhaplotype may increase susceptibility to HIV-1 infection. However, this
\ndifference did not reach statistical significance (p: 0.26; OR: 1.63; CI of OR (\n0,73-3,68). Similar results were also found for the 2 other haplotypes.\nTherefore, we conclude that that the three IL10 SNPs haplotypes are not\nassociated with HIV-1 susceptibility in our population, suggesting that other\nSNPs and or genes may be important for HIV-1 acquisition in Mali. <\/p>\n\n\n\n

D Dabitao, M. Dembele, J. Bream,\nB. Kone, M. Wague, C. Nadie, YS Sarro, B. Baya, D. Goita, S. Dao\u2019, R Murphy,\nWR. Bishai, S. Doumbia and S. Diallo.<\/strong><\/p>\n\n\n\n

\u00b0 University Clinical Research Center (UCRC) <\/p>\n\n\n\n

\u00b0 Johns Hopkins University (JHU) <\/p>\n\n\n\n

\u00b0 National Institutes of Allergy and Infectious Diseases (NIAID)<\/p>\n\n\n\n

\u00b0 Northwestern University (NU)<\/p>\n","protected":false},"excerpt":{"rendered":"

During the \u201cGenetics of Infectious Diseases\u201d session of the 12th Conference of the AfSHG and the 1st MSHG, Dr. Djeneba Dabitao, presented the topic: \u201cGenetic variations in human IL10 proximal promoter and Susceptibility to HIV infection: A pilot study in Mali, West Africa\u201d. Background: Interleukin-10 (IL-10) is a key anti-inflammatory cytokine…<\/p>\n","protected":false},"author":2,"featured_media":934,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-939","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-activities"],"_links":{"self":[{"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/posts\/939","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=939"}],"version-history":[{"count":1,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/posts\/939\/revisions"}],"predecessor-version":[{"id":940,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/posts\/939\/revisions\/940"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=\/wp\/v2\/media\/934"}],"wp:attachment":[{"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=939"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=939"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ucrc-mali.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=939"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}